Critical reflections on synthetic gene design for recombinant protein expression

Gene Expression Systems and Recombinant Protein Purification

There is no substance as important as deoxyribonucleic acid as it carries within its own structure the hereditary in formations that determines the structure of proteins, the important molecule of life. Deoxyribonucleic acid transcribe messenger ribonucleic acid which finally translates protein the essential macromolecules. Through gene expression the information contained in gene can be utiliz...

EuGene: maximizing synthetic gene design for heterologous expression

UNLABELLED Numerous software applications exist to deal with synthetic gene design, granting the field of heterologous expression a significant support. However, their dispersion requires the access to different tools and online services in order to complete one single project. Analyzing codon usage, calculating codon adaptation index (CAI), aligning orthologs and optimizing genes are just a fe...

Advances and challenges in recombinant protein expression in Escherichia coli

Critical Reflections on Regulation

Expression of Recombinant Protein B Subunit Pili from Vibrio Cholera

Background & Aims: Vibrio cholerae is a gram-negative bacterial pathogen that causes cholera disease. Following ingestion by a host and entry into the upper intestine, V. cholera colonizes and begins to emit enterotoxin. One of the most pathogenic factors of Vibrio cholera is toxin-coregulated pili (TCP). ToxinCoregulated pili is as the primary factor requiered for the colonization and insisten...